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KMID : 0378619950120010207
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Baptist Hospital Medical Journal
1995 Volume.12 No. 1 p.207 ~ p.214
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Effects of Retinoic Acid on the Development of Squamous Metaplasia for Rat Mammary Epithelial Cells Cultured in Matrigel
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Abstract
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Mammary organoids(ductal and endbud fragments) were cultured in a serum free medium (SFM) with/without 5% FBS, EGF, insulin, hydrocortisone, and human transferrin. Several types of colonies were observed : stellate(14±5.5%), duct(41±5.6%),
web(35±5.6%), squamous(6±2.1%), and lobuloduct(4±1.2%). Squamous colony was typical squamous metaplasia(SM) with several layers of squamous epithelia and keratin pearls. At the immunocytochemical study, casein proteins were predominantly
localized
near the apical surfaces of the cells or in the lumina of ductal or lobuloductal colonies. To inhibit the forination of SM, we treated organoids with all-trans retinoic acid(RA) from 10E-6 to 10E-17 M in SFM. Formation of SM was not completely
inhibited
in SFM. However, it was completely inhibited at 10E-9 M RA in CHM with 5% FBS.
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KEYWORD
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